• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文OA文献 >Antisense Suppression of 2-Cysteine Peroxiredoxin in Arabidopsis Specifically Enhances the Activities and Expression of Enzymes Associated with Ascorbate Metabolism But Not Glutathione Metabolism1
【2h】

Antisense Suppression of 2-Cysteine Peroxiredoxin in Arabidopsis Specifically Enhances the Activities and Expression of Enzymes Associated with Ascorbate Metabolism But Not Glutathione Metabolism1

机译：反义抑制2-半胱氨酸过氧化物酶在拟南芥中特别增强了抗坏血酸代谢而不是谷胱甘肽代谢相关酶的活性和表达1

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The aim of this study was to characterize the effect of decreased 2-cysteine peroxiredoxin (2-CP) on the leaf anti-oxidative system in Arabidopsis. At three stages of leaf development, two lines of transgenic Arabidopsis mutants with decreased contents of chloroplast 2-CP were compared with wild type and a control line transformed with an empty vector. Glutathione contents and redox state were similar in all plants, and no changes in transcript levels for enzymes involved in glutathione metabolism were observed. Transcript levels for chloroplastic glutathione peroxidase were much lower than those for 2-CP, and both cytosolic and chloroplastic glutathione peroxidase were not increased in the mutants. In contrast, the foliar ascorbate pool was more oxidized in the mutants, although the difference decreased with plant age. The activities of thylakoid and stromal ascorbate peroxidase and particularly monodehydroascorbate reductase were increased as were transcripts for these enzymes. No change in dehydroascorbate reductase activity was observed, and effects on transcript abundance for glutathione reductase, catalase, and superoxide dismutase were slight or absent. The results demonstrate that 2-CP forms an integral part of the anti-oxidant network of chloroplasts and is functionally interconnected with other defense systems. Suppression of 2-CP leads to increased expression of other anti-oxidative genes possibly mediated by increased oxidation state of the leaf ascorbate pool.
机译：这项研究的目的是表征减少的2-半胱氨酸过氧化物酶（2-CP）对拟南芥叶片抗氧化系统的影响。在叶片发育的三个阶段，将两株叶绿体2-CP含量降低的转基因拟南芥突变体与野生型进行比较，并将对照系转化为空载体。在所有植物中，谷胱甘肽含量和氧化还原状态相似，并且未观察到与谷胱甘肽代谢有关的酶的转录水平变化。叶绿体谷胱甘肽过氧化物酶的转录水平远低于2-CP，而突变体中胞质和叶绿体谷胱甘肽过氧化物酶的转录水平均未增加。相比之下，叶突变型抗坏血酸库在突变体中被更多地氧化，尽管随着植株的年龄差异有所减少。类囊体和间质抗坏血酸过氧化物酶，特别是单脱氢抗坏血酸还原酶的活性增加，这些酶的转录物也一样。没有观察到脱氢抗坏血酸还原酶活性的变化，对谷胱甘肽还原酶，过氧化氢酶和超氧化物歧化酶对转录本丰度的影响微乎其微。结果表明，2-CP构成叶绿体抗氧化剂网络的组成部分，并且在功能上与其他防御系统互连。 2-CP的抑制导致其他抗氧化基因的表达增加，可能是由叶片抗坏血酸盐池的氧化状态增加所介导的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号